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Microfluidic Chip-Based Induced Phase Separation Extraction as a Fast and Efficient Miniaturized Sample Preparation

Yao Shen^1,2, Bo Chen¹, Han Zuilhof²

¹Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research, Ministry of Education, Hunan Normal University, Changsha 410081, China.

Molecules (Basel, Switzerland)

|December 30, 2020

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This summary is machine-generated.

This study miniaturized induced phase separation extraction (IPSE) using a microfluidic chip, offering efficient sample purification with reduced consumption. The novel IPSE chip successfully separated complex mixtures, demonstrating high extraction efficiency.

Area of Science:

Analytical Chemistry
Microfluidics
Separation Science

Background:

Liquid-liquid extraction (LLE) is a common but often inefficient sample clean-up technique.
Induced phase separation extraction (IPSE) offers an efficient alternative to LLE.
Miniaturization of IPSE can lead to reduced sample and solvent consumption.

Purpose of the Study:

To miniaturize induced phase separation extraction (IPSE) by implementing it on a microfluidic chip.
To evaluate the efficiency of a microfluidic IPSE chip for sample separation and purification.
To demonstrate the application of microscale IPSE for complex sample matrices.

Main Methods:

Design and fabrication of a 5 × 2 cm microfluidic chip with 100 µm wide and 40 µm deep channels.

Keywords:

IPSE Scutellaria baicalensis TCM fast sample clean-up green analytical chemistry low solvent consumption microfluidics miniaturization on-chip separation μLPME

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Introduction of a sample solution (model compounds in acetonitrile-water) and a hydrophobic solvent inducer into the chip.

Phase separation within microchannels, allowing analytes to partition into favorable phases.

Adjustment of sample pH to influence the partitioning of pH-dependent analytes.

Application of the microfluidic IPSE chip to separate aglycones and glycosides from *Scutellaria baicalensis* extract.

Main Results:

Efficient separation of polar to non-polar model compounds into two immiscible phases within the microchip.
Successful fractionation of analytes based on their phase preferences.
Demonstrated control over analyte partitioning by adjusting the sample solution's pH.
Achieved high extraction efficiency, up to 98.8%, with no emulsion formation.
Successfully separated aglycones and glycosides from a Traditional Chinese Medicine (*Scutellaria baicalensis*) extract.

Conclusions:

Microfluidic induced phase separation extraction (IPSE) is a highly efficient and miniaturized sample clean-up technique.
The developed IPSE chip enables rapid (less than one minute) and effective sample pretreatment with minimal sample and solvent usage (microliters).
Microscale IPSE is a versatile tool applicable to complex natural product extracts and offers advantages over traditional LLE.