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Related Experiment Video

Updated: Nov 23, 2025

Core/shell Printing Scaffolds For Tissue Engineering Of Tubular Structures
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Core/shell Printing Scaffolds For Tissue Engineering Of Tubular Structures

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Capillary Networks for Bio-Artificial Three-Dimensional Tissues Fabricated Using Cell Sheet Based Tissue Engineering.

Hidekazu Sekine1, Teruo Okano2,3

  • 1Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo 162-8666, Japan.

International Journal of Molecular Sciences
|December 30, 2020
PubMed
Summary

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Author Correction: Densely vascularized thick 3D tissue shows enhanced protein secretion constructed with intermittent positive pressure.

Communications biology·2025
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Densely vascularized thick 3D tissue shows enhanced protein secretion constructed with intermittent positive pressure.

Communications biology·2025

Researchers developed a new method for creating thick, vascularized tissues for regenerative medicine. This technique uses stacked cell sheets on a vascular bed to form capillary networks, enabling functional tissue transplantation.

Area of Science:

  • Regenerative Medicine
  • Tissue Engineering
  • Vascular Biology

Background:

  • Developing vascular networks in bioengineered tissues is crucial for regenerative medicine.
  • Existing methods like angiogenic factor release or microchannel scaffolds are insufficient for thick tissue vascularization.
  • Co-culturing graft tissue with vascular cells shows promise for promoting capillary angiogenesis.

Purpose of the Study:

  • To develop a method for constructing thick, vascularized tissues suitable for transplantation.
  • To create a continuous circulatory structure within bioengineered tissues.
  • To overcome the limitations of current vascularization strategies.

Main Methods:

  • Progressive stacking of three-layered cell sheets onto a pre-formed vascular bed.
Keywords:
bioreactorcell sheet technologyregenerative medicinetissue culturetissue engineeringvascular bedvascularization

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  • Induction of capillary network formation within the stacked cell sheets.
  • Utilizing the vascular bed's artery and vein for anastomosis with host vasculature.

    • Main Results:

    • Successful formation of capillary networks within the engineered cell sheets.
    • Construction of thick, high-density, functional tissue constructs.
    • Demonstrated feasibility of transplanting engineered tissue via vascular anastomosis.

    Conclusions:

    • The stacked cell sheet approach on a vascular bed is a viable strategy for creating vascularized engineered tissues.
    • This method overcomes the limitations of previous techniques for vascularizing thick tissues.
    • The engineered tissues are suitable for transplantation, offering potential advancements in regenerative medicine.