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Stem Cells and Extrusion 3D Printing for Hyaline Cartilage Engineering
Océane Messaoudi1, Christel Henrionnet1, Kevin Bourge1
1UMR 7365 CNRS-UL, IMoPA (Ingénierie Moléculaire et Physiopathologie Articulaire), Biopôle de l'Université de Lorraine, Campus Brabois-Santé, 9, Avenue de la Forêt de Haye, BP20199, 54505 Vandœuvre-Lès-Nancy, France.
Cells
|December 30, 2020
Summary
Cartilage tissue engineering using 3D bioprinting with mesenchymal stem cells (MSCs) offers a promising solution for cartilage defects. This approach aims to regenerate hyaline cartilage, preventing osteoarthritis development.
Area of Science:
- Regenerative Medicine
- Biomaterials Science
- Orthopedics
Background:
- Hyaline cartilage lacks self-healing capacity, making early treatment of focal lesions crucial to prevent osteoarthritis.
- Current surgical methods for cartilage repair are insufficient, highlighting the need for advanced regenerative strategies.
- Cartilage tissue engineering presents a viable alternative, leveraging new technologies for personalized medicine.
Purpose of the Study:
- To review mesenchymal stem cells (MSCs) sources, origins, and densities for extrusion-based bioprinting (EBB) in cartilage tissue engineering.
- To discuss bioink components and their benefits in creating cartilage substitutes.
- To explore EBB as an alternative to chondrocytes for hyaline cartilage reconstruction.
Main Methods:
- Review of literature on MSCs and bioinks for extrusion-based bioprinting.
- Analysis of different MSC sources (bone marrow, synovium, dental pulp, Wharton's jelly) and their chondrogenic potential.
- Evaluation of bioink formulations for mimicking native cartilage structure and function.
Main Results:
- Mesenchymal stem cells (MSCs) demonstrate significant potential for cartilage regeneration due to their proliferation and differentiation capabilities.
- Extrusion-based bioprinting (EBB) allows for layer-by-layer deposition of cell-seeded bioinks, enabling the mimicry of hyaline cartilage's zonal organization.
- Various bioink constituents can be utilized to develop functional cartilage substitutes.
Conclusions:
- Mesenchymal stem cells (MSCs) are suitable alternatives to chondrocytes for cartilage tissue engineering via extrusion-based bioprinting (EBB).
- The selection of appropriate MSC sources, densities, and bioink compositions is critical for successful cartilage regeneration.
- 3D bioprinting holds significant promise for developing personalized, off-the-shelf cartilage repair solutions.
