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Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays
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Predicting Dimensions in Microfluidic Paper Based Analytical Devices.

Raquel Catalan-Carrio1,2, Tugçe Akyazi1, Lourdes Basabe-Desmonts2,3,4,5

  • 1Microfluidics Cluster UPV/EHU, Analytical Microsystems & Materials for Lab-on-a-Chip (AMMa-LOAC) Group, Analytical Chemistry Department, University of the Basque Country UPV/EHU, 01006 Vitoria-Gasteiz, Spain.

Sensors (Basel, Switzerland)
|December 30, 2020
PubMed
Summary
This summary is machine-generated.

Optimizing the heating process in wax printing is key to reproducible microfluidic paper analytical devices. This study analyzes critical parameters to ensure consistent device dimensions for reliable fluid flow control.

Keywords:
LOCpaper microfluidicspaper microfluidics fabricationwax printingµPAD

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Area of Science:

  • Microfluidics
  • Analytical Chemistry
  • Materials Science

Background:

  • Microfluidic paper-based analytical devices (µPADs) offer potential for low-cost diagnostics but face challenges in mass production due to poor fluid flow control.
  • Uncontrolled fabrication protocols, particularly in wax printing, lead to inconsistent device dimensions and unreliable performance.

Purpose of the Study:

  • To optimize the wax printing fabrication process for microfluidic paper-based analytical devices.
  • To identify and analyze key parameters affecting device dimensions and reproducibility.
  • To develop a method for predicting and controlling the working areas of paper-based devices.

Main Methods:

  • Systematic optimization of the heating step in wax printing.
  • Analysis of fabrication parameters including shape, wax barrier width, and internal device area.
  • Development of a predictive model for device dimensions.

Main Results:

  • Identified critical heating parameters for reproducible fabrication of microfluidic channels.
  • Demonstrated that device shape, barrier width, and internal area significantly influence final dimensions.
  • Presented a straightforward method to achieve predictable and controlled working areas in paper-based devices.

Conclusions:

  • Optimized heating protocols are essential for reproducible microfluidic paper-based analytical devices.
  • Precise control over fabrication parameters enables reliable fluid flow and consistent device performance.
  • The developed method facilitates the mass production and expansion of paper-based analytical devices.