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Updated: Nov 23, 2025
08:11
Quantitative Methods to Study Protein Arginine Methyltransferase 1-9 Activity in Cells
Published on: August 7, 2021
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The uncharacterized protein FAM47E interacts with PRMT5 and regulates its functions
Baskar Chakrapani1, Mohd Imran K Khan1, Rajashekar Varma Kadumuri2
1Department of Biotechnology, Pondicherry University, Puducherry, India.
Life Science Alliance
|December 30, 2020
Summary
Researchers discovered FAM47E, a novel protein that interacts with Protein Arginine Methyltransferase 5 (PRMT5). FAM47E stabilizes PRMT5, enhancing its cancer-associated functions, suggesting a new therapeutic target for cancer treatment.
Area of Science:
- Molecular Biology
- Epigenetics
- Cancer Research
Background:
- Protein arginine methyltransferase 5 (PRMT5) plays a crucial role in various cellular processes and is implicated in cancer development and progression.
- Elevated PRMT5 levels correlate with poor clinical outcomes in several cancers, highlighting its significance as a therapeutic target.
Purpose of the Study:
- To identify novel interaction partners of PRMT5.
- To elucidate the functional role of FAM47E in regulating PRMT5 activity and its implications in cancer.
Main Methods:
- Yeast two-hybrid screening to identify PRMT5 interacting proteins.
- Immunoprecipitation and pull-down assays to confirm protein-protein interactions.
- Assays to assess PRMT5 stability, chromatin association, histone methylation activity, and gene expression.
Main Results:
- FAM47E was identified as a novel interaction partner of PRMT5.
- FAM47E stabilizes PRMT5 by inhibiting its proteasomal degradation, thereby increasing its steady-state levels.
- FAM47E enhances PRMT5's chromatin association and histone methylation activity, promoting PRMT5-mediated gene regulation and cellular transformation.
Conclusions:
- FAM47E acts as a positive regulator of PRMT5, enhancing its oncogenic functions.
- Targeting the PRMT5-FAM47E interaction with small molecules presents a potential therapeutic strategy to inhibit PRMT5's role in cancer.
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