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Expression of TIPE family members in human colorectal cancer
Mengya Zhong1, Zhijian Chen1, Yang Yan1
1Cancer Research Center, School of Medicine, Xiamen University, Xiamen, Fujian 361005, P.R. China.
Abstract:
The tumor necrosis factor α-induced protein 8 (TNFAIP8)-like (TIPE) protein family comprises four members, namely TNFAIP8, TIPE1, TIPE2 and TIPE3, which are involved in multiple processes in cancer. The current study aimed to investigate the expression patterns and potential clinical roles of the TIPE family members in human colorectal cancer (CRC). Paired tumor and adjacent tissue samples were collected from 49 patients with CRC, and the relative mRNA expression levels of the TIPE family members in these samples were evaluated by using reverse transcription-quantitative PCR, and the protein levels in five randomly selected pairs of tumor and adjacent tissue samples were detected by western blot analysis. The mRNA expression levels of the TIPE family members were significantly downregulated in CRC tumor tissues compared with those in the adjacent tissues; however, within each sample, TNFAIP8 and TIPE3 protein levels were only partially consistent with their mRNA levels. In addition, the mRNA expression levels between each pair of TIPE family members exhibited a positive linear relationship, and TIPE2 mRNA levels exhibited strong linear associations with those of TNFAIP8 and TIPE1. TNFAIP8 mRNA expression levels in tumor tissues were significantly associated with the tumor differentiation grade, and TIPE2 mRNA expression levels in tumor tissues were significantly associated with sex. TIPE1 and TIPE3 mRNA expression levels in tumor tissues exhibited no associations with patient clinicopathological characteristics. In addition, the mRNA expression patterns of the TIPE family members were analyzed using data from The Cancer Genome Atlas data set, and the results also demonstrated that TNFAIP8, TIPE2 and TIPE3 mRNA levels were downregulated in patients with colon adenocarcinoma compared with those in normal controls. These results provided evidence that the four members of the TIPE family may affect each other to mediate the carcinogenesis of CRC, and that TIPE2 may serve an important role in CRC.
Insights
The tumor necrosis factor α-induced protein 8 (TNFAIP8)-like (TIPE) protein family members are downregulated in colorectal cancer (CRC). TIPE2 may play a significant role in CRC development and progression.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- The tumor necrosis factor α-induced protein 8 (TNFAIP8)-like (TIPE) protein family, comprising TNFAIP8, TIPE1, TIPE2, and TIPE3, is implicated in various cancer processes.
- Understanding the expression and clinical significance of TIPE family members in colorectal cancer (CRC) is crucial for potential therapeutic strategies.
Purpose of the Study:
- To investigate the expression patterns of TIPE family members in human colorectal cancer (CRC).
- To explore the potential clinical roles and associations of TIPE family members with clinicopathological characteristics in CRC patients.
Main Methods:
- Quantitative analysis of TIPE family mRNA expression using reverse transcription-quantitative PCR in paired tumor and adjacent tissues from 49 CRC patients.
- Western blot analysis to detect protein levels of TNFAIP8 and TIPE3 in selected tissue samples.
- Analysis of The Cancer Genome Atlas (TCGA) dataset for TIPE family mRNA expression in colon adenocarcinoma.
Main Results:
- All four TIPE family members (TNFAIP8, TIPE1, TIPE2, TIPE3) showed significantly downregulated mRNA expression in CRC tumor tissues compared to adjacent tissues.
- TNFAIP8 and TIPE3 protein levels showed partial consistency with their mRNA levels.
- TIPE2 mRNA levels demonstrated strong linear associations with TNFAIP8 and TIPE1 mRNA levels.
- TNFAIP8 mRNA expression correlated with tumor differentiation grade, and TIPE2 mRNA expression correlated with patient sex.
- TCGA data confirmed downregulation of TNFAIP8, TIPE2, and TIPE3 mRNA in colon adenocarcinoma compared to normal controls.
Conclusions:
- The TIPE family members may interact and collectively influence colorectal cancer (CRC) carcinogenesis.
- TIPE2, in particular, may play a significant role in the development and progression of CRC.
- Downregulation of TIPE family members suggests a potential tumor-suppressive role in CRC.
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