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Characterization of the genomic sequence data around common cutworm resistance genes in soybean (Glycine max) using

Eri Ogiso-Tanaka¹, Nobuhiko Oki², Tsuyoshi Tanaka¹

¹Institute of Crop Science, National Agriculture and Food Research Organization (NARO), 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8518, Japan.

|December 30, 2020

Abstract:

The common cutworm (CCW, Spodopteraab litura Fabricius) is one of the pests that most severely infect soybean (Glycine max L. Merr.). In a previous report, quantitative trait loci (QTL) analysis of CCW resistance using a recombinant inbred line derived from a cross between a susceptible cultivar 'Fukuyutaka' and a resistant cultivar 'Himeshirazu', identified two antixenosis resistance QTLs, CCW-1 and CCW-2. To reveal sequence variation between the aforementioned two cultivars, whole genome resequencing was performed using Illumina HiSeq2000 (75,632,747 and 91,540,849 reads). The generated datasets can be used for fine mapping and gene isolation of CCW-1 and CCW-2 as well as for revealing more detailed genetic differences between 'Fukuyutaka' and 'Himeshirazu' .

Keywords:

Glycine max, Soybean HiSeq Oxford Nanopore MinION QTL region Resistance to the common cutworm Targeted amplicon sequencing Whole genome resequencing

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