Protocol for Co-culture of Microglia with Axons

Microglia, the resident immune cells of the central nervous system, accumulate along axons and support neuronal survival during the early postnatal period. Although methods have been developed to isolate microglia or neurons, the procedures for co-culture of these cells can inadvertently affect the interactions between them. Here, we describe a protocol to investigate the accumulation of microglia toward neuronal axons using axon isolation culture devices. This approach is useful for modeling neuron-glia associations. For complete details on the use and execution of this protocol, please refer to Ueno et al. (2013) and Fujita et al. (2020).