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Updated: Nov 23, 2025

Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube
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Protocol for Evaluating Neuronal Polarity in Murine Models.

Carlos Wilson1,2,3, Victoria Rozés-Salvador2,3, Alfredo Cáceres1,2,3

  • 1Centro de Investigación en Medicina Traslacional "Severo R Amuchástegui" (CIMETSA), Instituto Universitario Ciencias Biomédicas Córdoba (IUCBC), Naciones Unidas 420, 5016 Córdoba, Argentina.

STAR Protocols
|December 30, 2020
PubMed
Summary
This summary is machine-generated.

This study details methods for observing neuron polarity acquisition in rat hippocampal cultures and mouse brains. These techniques aid in exploring neuronal development, growth, and disease mechanisms.

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Area of Science:

  • Neuroscience
  • Developmental Biology
  • Cell Biology

Background:

  • Neuronal polarity is essential for proper brain function and connectivity.
  • Understanding axo-dendritic specification is key to studying neural development and disease.

Purpose of the Study:

  • To provide detailed protocols for studying neuronal polarity acquisition.
  • To enable exploration of mechanisms and signals governing neural development and disease.

Main Methods:

  • Utilizing primary cultures of embryonic rat hippocampal neurons for in vitro analysis.
  • Employing in utero electroporation of GFP-expressing embryonic mouse brains for in situ visualization of cortical neuron migration and polarization.
  • Including underreported post-protocol steps for comprehensive methodology.

Main Results:

  • Established protocols for in vitro and in situ monitoring of neuronal polarity.
  • Demonstrated visualization of cortical neuron migration and polarization.

Conclusions:

  • The described methods offer a robust framework for investigating neuronal polarity.
  • These techniques facilitate research into neural development, growth, and associated diseases.