Making Droplet-Embedded Vesicles to Model Cellular Lipid Droplets
Related Experiment Video
Updated: Nov 23, 2025

Lipid Bilayer Vesicle Generation Using Microfluidic Jetting
Published on: February 21, 2014
Aymeric Chorlay1, Alexandre Santinho1, Abdou Rachid Thiam1
1Laboratoire de Physique de l'École Normale Supérieure, ENS, Université PSL, CNRS, Sorbonne Université, Université de Paris, 75005 Paris, France.
Abstract:
We present a reproducible protocol to prepare droplet-embedded vesicles (DEVs) consisting of an oil droplet embedded within a phospholipid bilayer. This model system mimics a cellular lipid droplet (LD) in physical contact with the endoplasmic reticulum (ER) bilayer. It has the advantage that the lipid composition and the biophysical properties of the droplet and the bilayer are controlled and tunable. DEVs can be used to study LD biogenesis factors and determinants of protein binding between ER and LD interfaces. For complete details on the use and execution of this protocol, please refer to Chorlay and Thiam (2020) and Santinho et al. (2020).
Related Concept Videos
Pinching-off of Coated Vesicles
COP Coated Vesicles
Intralumenal Vesicles and Multivesicular Bodies

