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Updated: Nov 23, 2025
Production of Lentiviral Vectors for Transducing Cells from the Central Nervous System
Published on: May 24, 2012
An Improved Protocol for the Production of Lentiviral Vectors
Logan Y Brown1, Wendy Dong1, Boris Kantor1,2
1Duke Viral Vector Core, Department of Neurobiology, Duke University Medical Center, Durham, NC 27710, USA.
Abstract:
Lentiviral vectors are an ideal gene-delivery system for large gene-editing tools, such as the clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 system, due to their high packaging capacity and broad tropism. Here, we present a calcium phosphate-based protocol for lentiviral production and concentration for in vitro and in vivo use. This revised procedure has been optimized to ensure high viral titers and transduction efficiency and is scalable to meet specific production needs.
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