Related Experiment Video
Updated: Jun 14, 2026
14:02
Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons
Published on: October 31, 2020
Protocol for BATTLE-1EX: A High-Resolution Imaging Method to Visualize Whole Synaptic Structures and their Components
Akitoshi Inoue1, Takuya Kobayashi1, Hirokazu Hirai2,3
1Department of Medical Chemistry, Kansai Medical University, Graduate School of Medicine, Hirakata, Osaka 573-1010, Japan.
STAR Protocols
|December 30, 2020
Summary
BATTLE-1EX combines BATTLE-1 and expansion microscopy for high-resolution imaging of synaptic structures in neural circuits. This method allows detailed visualization and dissection of synapses within the brain.
Area of Science:
- Neuroscience
- Molecular Biology
- Microscopy
Background:
- Understanding neural circuits requires high-resolution imaging of synaptic structures.
- Existing methods may have limitations in visualizing entire synaptic components.
Purpose of the Study:
- To describe BATTLE-1EX, a novel combined method for high-resolution imaging of synaptic structures.
- To enable detailed visualization and dissection of synaptic components in hippocampal neural circuits.
Main Methods:
- BATTLE-1EX integrates BATTLE-1 (using titratable recombinase competition) with expansion microscopy.
- This approach allows for tunable proportions of specific cell populations (mCherry+/YFP- and YFP+/mCherry-).
Main Results:
- BATTLE-1EX provides high-resolution imaging of whole synaptic structures.
- The method facilitates the visualization and dissection of synaptic components in neural circuits.
Conclusions:
- BATTLE-1EX offers a powerful approach for studying synaptic organization and function.
- This combinational method has broad potential for neuroscience research in various brain regions.
