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Updated: Nov 23, 2025

Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus
Published on: July 27, 2021
Replication-Independent Generation and Morphological Analysis of Flavivirus Replication Organelles
Sarah Goellner1, Berati Cerikan1, Mirko Cortese1
1Department of Infectious Diseases, Molecular Virology, University of Heidelberg, Heidelberg, 69120, Germany.
Abstract:
Positive-strand RNA viruses replicate in distinct membranous structures called replication organelles (ROs). Mechanistic studies of RO formation have been difficult because perturbations affecting viral replication have an impact on viral protein amounts, thus affecting RO biogenesis. Here, we present a detailed guide on how to use a replication-independent expression system, designated pIRO (plasmid-induced replication organelle formation), inducing bona fide flavivirus ROs in transfected cells. This will be useful for mechanistic studies of viral and cellular factors driving flavivirus RO biogenesis. For complete details on the use and execution of this protocol, please refer to Cerikan et al. (2020).
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