Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

tRNA methylation: functional insights and epitranscriptomic regulation.

Cell communication and signaling : CCS·2026
Same author

Oncologic outcomes of transurethral resection versus laser excision for non-muscle invasive bladder cancer: a SEER retrospective cohort study.

Translational andrology and urology·2026
Same author

Machine learning and neural network algorithms for prediction of C5 palsy after posterior surgery for ossification of posterior longitudinal ligament.

European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·2026
Same author

From repair to disease: lymphatic contributions to regeneration, cancer and ageing.

Journal of advanced research·2026
Same author

Asymmetric histone inheritance regulates olfactory stem cell fates during regeneration.

Nature communications·2026
Same author

Wnt-presenting materials sustain H3K14-acetylation in human skeletal stem cells for tissue engineering and bone repair.

NPJ Regenerative medicine·2026

Related Experiment Video

Updated: Nov 23, 2025

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C
09:32

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C

Published on: October 14, 2022

4.1K

Protocol for Establishing Mouse Embryonic Stem Cells to Study Histone Inheritance Pattern at Single-Cell Resolution.

Binbin Ma1, Tung-Jui Trieu2, Shukry J Habib2

  • 1Department of Biology, The Johns Hopkins University, Baltimore, MD 21218, USA.

STAR Protocols
|December 30, 2020
PubMed
Summary

Asymmetric histone inheritance in mouse embryonic stem cells (mESCs) was studied. This protocol enables tracking histone patterns during cell division for future research.

Keywords:
Cell BiologySingle CellStem Cells

More Related Videos

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark
10:09

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark

Published on: January 26, 2018

7.7K
Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos
11:02

Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos

Published on: April 29, 2011

18.3K

Related Experiment Videos

Last Updated: Nov 23, 2025

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C
09:32

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C

Published on: October 14, 2022

4.1K
Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark
10:09

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark

Published on: January 26, 2018

7.7K
Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos
11:02

Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos

Published on: April 29, 2011

18.3K

Area of Science:

  • Cell Biology
  • Epigenetics
  • Developmental Biology

Background:

  • Asymmetric histone inheritance regulates cell fate in *Drosophila* male germline stem cells.
  • The role of asymmetric histone inheritance in mammalian systems is not well understood.
  • Previous work showed distinct H3/H4 patterns in mouse embryonic stem cells (mESCs) near Wnt3a beads.

Purpose of the Study:

  • To present a detailed protocol for tracking histone inheritance in asymmetrically dividing mESCs.
  • To establish a new system for studying histone inheritance in cultured mammalian cells.
  • To enable the application of this method to other cell systems.

Main Methods:

  • Utilizing mouse embryonic stem cells (mESCs).
  • Employing Wnt3a beads to induce asymmetric division.
  • Developing a single-cell resolution protocol for tracking histone H3/H4 patterns.

Main Results:

  • Demonstrated non-overlapping H3/H4 patterns in asymmetrically dividing mESCs.
  • Established a method for high-resolution analysis of histone inheritance.

Conclusions:

  • The developed protocol provides a novel system for investigating histone inheritance in mammalian cell culture.
  • This research opens avenues for studying epigenetic regulation of cell fate in mammals.
  • The protocol can be adapted for use in various parallel biological systems.