Related Experiment Video
Updated: Nov 23, 2025
Preparing Lamellae from Vitreous Biological Samples Using a Dual-Beam Scanning Electron Microscope for Cryo-Electron Tomography
Published on: August 5, 2021
Protocol for Extraction and Electron Microscopy Visualization of Lipids in Viburnum tinus Fruit Using
Miranda Sinnott-Armstrong1, Silvia Vignolini2, Yu Ogawa3
1Department of Ecology and Evolutionary Biology, Yale University, New Haven, CT 06511, USA.
Abstract:
We recently reported that Viburnum tinus fruit generates its metallic blue color using globular lipid inclusions embedded in its epicarpal cell walls. This protocol describes steps to visualize the lipidic nature of the nanostructure using cryo-ultramicrotomy, chloroform extraction, and transmission electron microscopy (TEM) imaging. This method is useful to localize and characterize novel lipidic nanostructures embedded in both plant and animal tissues at the TEM resolution. For complete details on the use and execution of this protocol, please refer to Middleton et al. (2020).
More Related Videos
11:45The CryoAPEX Method for Electron Microscopy Analysis of Membrane Protein Localization Within Ultrastructurally-Preserved Cells
Published on: February 27, 2020
13:35Plunge Freezing: A Tool for the Ultrastructural and Immunolocalization Studies of Suspension Cells in Transmission Electron Microscopy
Published on: May 5, 2017
Related Concept Videos
01:28Cryo-electron Microscopy
01:20Preparation of Samples for Electron Microscopy
01:03Fixation and Sectioning
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...