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Large-Scale SARS-CoV-2 Testing Utilizing Saliva and Transposition Sample Pooling

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Informed sequential pooling approach to detect SARS-CoV-2 infection.

Renato Millioni¹, Cinzia Mortarino²

¹Fascial Manipulation Institute by Stecco, Padova, Italy.

|December 30, 2020

Summary

A two-step pooling strategy can efficiently diagnose COVID-19 (coronavirus disease 2019) by testing pooled samples. This method significantly reduces costs and time for large-scale SARS-CoV-2 testing.

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Area of Science:

Virology
Epidemiology
Clinical Diagnostics

Background:

The COVID-19 pandemic caused by SARS-CoV-2 necessitates rapid and large-scale diagnostic testing.
Timely diagnosis, including testing asymptomatic individuals, is crucial for controlling SARS-CoV-2 spread.
Clinical laboratories require increased throughput for effective pandemic response.

Purpose of the Study:

To evaluate a two-step sequential pooling strategy for SARS-CoV-2 detection.
To assess the efficiency of pooled sample testing in reducing the number of diagnostic tests required.
To determine if pooling strategies can be customized based on cohort characteristics and virus frequency.

Main Methods:

Simulated diagnostic testing protocols using pooled samples.
Assessed efficiency based on the number of tests needed for random and "informed" pools.
Varied pool sizes and virus frequencies in different simulation scenarios.

Main Results:

The proposed two-step pooling procedure effectively identifies positive subjects.
Significant cost and time savings were demonstrated compared to individual testing.
Pooling strategies can be tailored based on epidemiological data and cohort specifics.

Conclusions:

A sequential pooling approach offers an efficient method for large-scale SARS-CoV-2 diagnostics.
This strategy enhances laboratory throughput while reducing resource utilization.
Customizable pooling enhances diagnostic efficiency according to specific population and disease prevalence characteristics.