A facile solvent extraction method facilitating surface-enhanced Raman spectroscopic detection of ochratoxin A in
Lourdes Martinez Rojas1, Yanqui Qu1, Lili He1
1Department of Food Science, University of Massachusetts, MA, Amherst, USA.
Abstract:
The capability of a solvent-mediated liquid-liquid extraction (LLE) method to improve the detection of ochratoxin A (OTA) in food matrixes using surface-enhanced Raman spectroscopy (SERS) is described. SERS detection of mycotoxins with nanoparticle aggregation is a simple method but with low reproducibility due to the heterogeneous distribution of the nanoparticle aggregates. We evaluated three different LLE protocols to analyze their performance in combination with SERS. A facile extraction method based on sample acidification and addition of chloroform as a separation solvent showed to not only extract OTA from wine and wheat but also facilitate the uniform distribution of the nanoparticles leading to an improvement of the detection signals and the reproducibility. This method enables rapid and simple analysis of mycotoxin Ochratoxin A in food systems.
More Related Videos
08:13A Filter-based Surface Enhanced Raman Spectroscopic Assay for Rapid Detection of Chemical Contaminants
Published on: February 19, 2016
11:59Extraction and Detection of Geosmin and 2-Methylisoborneol in Water and Fish using High-Capacity Sorptive Extraction Probes and GC-MS
Published on: July 3, 2025