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A smartphone-assisted portable biosensor using laccase-mineral hybrid microflowers for colorimetric determination of
Miaorong Zhang1, Yan Zhang2, Chuankai Yang1
1Institute of Hybrid Materials, National Center of International Joint Research for Hybrid Materials Technology, National Base of International Science & Technology Cooperation, College of Materials Science and Engineering, Qingdao University, Qingdao, 266071, People's Republic of China.
Abstract:
A novel smartphone-assisted portable biosensor based on laccase-mineral hybrid microflowers (La-HMFs) was applied for real-time, accurate and reliable quantification of epinephrine (EP). La-HMFs with flower-like hierarchical nanostructure was synthesized via biomineralization using Cu3(PO4)2⋅3H2O as the mineral. Characterization results revealed laccase molecules as the framework were immobilized within La-HMFs. Smartphone-assisted colorimetric assays showed wide linear detection range (1-400 μM) and favorable anti-interference ability for EP detection, and the detection limit was 0.6 μM. Further, due to the protective effect of Cu3(PO4)2⋅3H2O, the immobilized laccase exhibited good stability and desirable reusability.
