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Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells

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Yeast Translation Elongation Factor eIF5A Expression Is Regulated by Nutrient Availability through Different

Marina Barba-Aliaga^1,2, Carlos Villarroel-Vicente^1,2, Alice Stanciu^1,2

¹Instituto Biotecmed, Facultad de Ciencias Biológicas, Universitat de València, C/Dr. Moliner 50, E46100 Burjassot, Spain.

International Journal of Molecular Sciences

|December 31, 2020

Summary

Translation elongation factor eIF5A (eIF5A) expression is regulated by cellular metabolism. Yeast studies show Tif51A, an eIF5A isoform, is crucial for respiration and its expression adapts to nutrient availability.

Keywords:

Hap1 Snf1 TOR eIF5A gene expression heme iron mitochondrial respiration

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Published on: December 25, 2021

Area of Science:

Molecular Biology
Cellular Metabolism
Gene Regulation

Background:

eIF5A is essential for translation, particularly for proline-rich sequences.
Human eIF5A has two isoforms (eIF5A-1 and eIF5A-2) with differential roles in cancer.
eIF5A's connection to mitochondria and regulation by metabolism remains unclear.

Purpose of the Study:

To investigate the regulation of yeast eIF5A isoforms (Tif51A and Tif51B) by metabolic conditions.
To elucidate the role of eIF5A in mitochondrial function and respiration.

Main Methods:

Analysis of yeast Tif51A and Tif51B expression under various metabolic conditions (glucose levels, carbon sources).
Study of yeast mutants affecting metabolic signaling pathways (TORC1, Snf1) and iron availability.
Measurement of growth rates and oxygen consumption in response to Tif51A depletion.

Main Results:

Tif51A depletion impaired yeast growth and oxygen consumption during respiration.
Tif51A expression is positively regulated by high glucose (via TORC1) for growth.
Tif51A expression is also regulated by low glucose/non-fermentative carbon sources (via Snf1 and Hap1) for respiration.
Iron depletion led to Tif51A downregulation and Tif51B upregulation, both dependent on Hap1.

Conclusions:

Yeast eIF5A isoform expression is dynamically regulated by the cellular metabolic state.
Tif51A plays a critical role in supporting respiration, with its expression linked to nutrient availability and mitochondrial function.
Metabolic control of eIF5A provides a link between cellular energy status and protein synthesis efficiency.