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Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

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Iterative optical diffraction tomography for illumination scanning configuration.

Shengli Fan, Seth Smith-Dryden, Guifang Li

|December 31, 2020

Summary

This summary is machine-generated.

Optical diffraction tomography (ODT) reconstructs refractive indices. A new iterative ODT for illumination scanning configuration (ISC) improves imaging of multiply-scattering biological samples, overcoming limitations of conventional methods.

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Area of Science:

Biomedical optics
Quantitative phase imaging
Optical microscopy

Background:

Optical diffraction tomography (ODT) reconstructs refractive index distributions using multiple measurements.
Illumination scanning configuration (ISC)-based ODT offers fast data acquisition and stability, making it suitable for biological imaging.
Conventional ODT struggles with multiply-scattering samples.

Purpose of the Study:

To adapt iterative ODT (iODT) for the illumination scanning configuration (ISC) to address limitations in imaging multiply-scattering samples.
To develop and numerically validate an ISC update for iterative ODT.
To compare the performance of the new iODT-ISC against conventional ODT.

Main Methods:

Development of an iterative ODT update specifically for the illumination scanning configuration (ISC).
Numerical demonstration of the accuracy and performance of the developed iODT-ISC.
Comparative analysis with conventional ODT using identical prior knowledge.

Main Results:

The developed iODT-ISC accurately reconstructs refractive index distributions for multiply-scattering samples.
iODT-ISC effectively resolves the missing-angle problem inherent in conventional ODT.
The new method demonstrates superior performance compared to conventional ODT under identical conditions.

Conclusions:

The novel iODT-ISC method significantly enhances the capability of ODT for imaging complex, multiply-scattering biological samples.
This advancement overcomes previous limitations of ODT in handling scattering media.
The developed ISC update provides a more robust approach for quantitative phase imaging in challenging biological specimens.