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[Quantitative analysis of DNA methylation by pyrosequencing and MassARRAY technique for age estimation: a comparative
Ling Wang1,2, Fuduan Peng3,4, Hui Zhao1
1Institute of Forensic Science, Ministry of Public Security//Ministry of Public Security Key Laboratory of Forensic Genetics, Beijing 100000, China.
Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University
|December 31, 2020
Summary
This study compared DNA methylation age estimation using MassARRAY and pyrosequencing. MassARRAY provided direct age prediction, while pyrosequencing required Z-score transformation to correct for increased error, improving age estimation accuracy.
Area of Science:
- Epigenetics
- Forensic Science
- Biotechnology
Background:
- DNA methylation patterns change with age, offering potential for age estimation.
- Quantitative methylation analysis techniques like MassARRAY and pyrosequencing are used for this purpose.
- Comparing these techniques is crucial for accurate age prediction.
Purpose of the Study:
- To compare the accuracy of age estimation using DNA methylation data obtained from MassARRAY and pyrosequencing.
- To evaluate the effectiveness of Z-score transformation in mitigating batch effects and improving age prediction accuracy for both techniques.
Main Methods:
- Analyzed methylation levels of 9 CpG sites in two whole blood sample sets (65 and 62 samples).
- Employed MassARRAY and pyrosequencing for DNA methylation detection.
- Utilized Z-score transformation to correct for technical batch effects and linear regression for age prediction.
Main Results:
- MassARRAY showed consistent age prediction accuracy (MAD ~2.4-3.4 years) before and after Z-score transformation.
- Pyrosequencing showed higher initial age prediction error (MAD ~3.6-4.2 years), significantly improved by Z-score transformation (MAD ~2.7-3.6 years).
- Z-score transformation effectively reduced system batch effects between the two techniques.
Conclusions:
- Z-score transformation is effective in reducing system batch effects between MassARRAY and pyrosequencing.
- MassARRAY allows direct age estimation from DNA methylation data.
- Pyrosequencing data requires Z-score transformation to correct for potential errors and improve age estimation accuracy.
