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Effects of LncRNA HCP5/miR-214-3p/MAPK1 Molecular Network on Renal Cell Carcinoma Cells

Jun-Feng Hao1, Pei Chen2, He-Yi Li3

  • 1Department of Nephrology and Blood Purification Center, Jin Qiu Hospital of Liaoning Province (Geriatric Hospital of Liaoning Province), Shenyang City, Liaoning Province 110000, People's Republic of China.

Cancer Management and Research
|December 31, 2020
PubMed
Abstract

Insights

Long non-coding RNA HCP5 promotes clear cell renal cell carcinoma (ccRCC) metastasis by regulating the miR-214-3p/MAPK1 axis. HCP5 may serve as a prognostic biomarker for ccRCC patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long non-coding RNAs (LncRNAs) are implicated in various carcinomas.
  • Clear cell renal cell carcinoma (ccRCC) metastasis is a major cause of cancer-related deaths.
  • The role of the LncRNA HCP5 in ccRCC remains largely unelucidated.

Purpose of the Study:

  • To investigate the expression and function of HCP5 in ccRCC.
  • To elucidate the underlying molecular mechanism of HCP5 in ccRCC progression.
  • To evaluate HCP5 as a potential biomarker for ccRCC prognosis.

Main Methods:

  • Real-time quantitative PCR (qRT-PCR) to assess HCP5 expression in ccRCC tissues.
  • Gain-of-function and loss-of-function assays to determine HCP5's biological effects on ccRCC cells.
  • RNA immunoprecipitation and Western blot analyses to explore the molecular mechanism involving miR-214-3p/MAPK1 axis.

Main Results:

  • HCP5 expression was significantly upregulated in ccRCC tissues and correlated with metastatic features.
  • Overexpression of HCP5 promoted ccRCC cell proliferation, migration, and invasion in vitro.
  • HCP5 deletion inhibited ccRCC cell growth and metastasis both in vitro and in vivo.
  • HCP5 was found to regulate the miR-214-3p/MAPK1 axis, thereby affecting ccRCC progression.

Conclusions:

  • HCP5 acts as a key LncRNA promoting ccRCC metastasis.
  • The HCP5/miR-214-3p/MAPK1 axis is a critical pathway in ccRCC development.
  • HCP5 holds potential as a prognostic biomarker for ccRCC.
Keywords:
HCP5MAPK1clear cell renal cell carcinomamiR-214-3p

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