Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Photoluminescence: Applications

Photoluminescence: Applications

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Donor Modulation of Strong Near-Infrared Lipid-Droplet Probes for in Vivo Diagnosis of Nonalcoholic Fatty Liver Disease.

Chemical & biomedical imaging·2026

Same author

Photocatalytic four-component reaction to access covalent organic frameworks for photocatalysis.

Nature communications·2026

Same author

Tandem reaction to access isoquinolone-linked covalent organic frameworks for photocatalytic synthesis of benzimidazoles.

Chemical communications (Cambridge, England)·2025

Same author

An engineered single-wavelength-excitable dual-color fluorescent probe for simultaneous imaging of polarity and viscosity dynamics during ferroptosis.

Analytica chimica acta·2025

Same author

Complex-mediated nucleophilic aromatic substitution with aryl nitriles to realize intramolecular flapping-restricted D-A AIEgens for bioimaging.

Smart molecules : open access·2025

Same author

Regioisomeric Engineering of Sterically Hindered Bright Near-Infrared Paraptosis Agents for Chemo-Photodynamic Therapy.

Journal of the American Chemical Society·2025

Search research articles

Related Experiment Video

Updated: Nov 23, 2025

Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy

Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy

Published on: June 7, 2019

Fluorescent AIE-Active Materials for Two-Photon Bioimaging Applications.

Qing Lu¹, Cheng-Juan Wu², Zhiqiang Liu¹

¹State Key Laboratory of Crystal Materials, and Advanced Medical Research Institute, Shandong University, Jinan, China.

Frontiers in Chemistry

|December 31, 2020

Summary

Aggregation-induced emission (AIE) materials offer superior two-photon fluorescence imaging for biological applications. These AIE materials provide enhanced visualization in live samples, overcoming limitations of traditional methods.

Keywords:

aggregation-induced emission fluorescence imaging fluorescent detection fluorescent material two-photon

More Related Videos

Two-Photon-Based Photoactivation in Live Zebrafish Embryos

Two-Photon-Based Photoactivation in Live Zebrafish Embryos

Published on: December 24, 2010

In Vivo Two-Color 2-Photon Imaging of Genetically-Tagged Reporter Cells in the Skin

In Vivo Two-Color 2-Photon Imaging of Genetically-Tagged Reporter Cells in the Skin

Published on: July 11, 2019

Related Experiment Videos

Last Updated: Nov 23, 2025

Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy

Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy

Published on: June 7, 2019

Two-Photon-Based Photoactivation in Live Zebrafish Embryos

Two-Photon-Based Photoactivation in Live Zebrafish Embryos

Published on: December 24, 2010

In Vivo Two-Color 2-Photon Imaging of Genetically-Tagged Reporter Cells in the Skin

In Vivo Two-Color 2-Photon Imaging of Genetically-Tagged Reporter Cells in the Skin

Published on: July 11, 2019

Area of Science:

Biomedical imaging
Materials science
Optics

Background:

Fluorescence imaging enables real-time visualization of biological processes with high selectivity and sensitivity.
Two-photon fluorescence imaging offers advantages like deep tissue penetration and reduced photodamage compared to one-photon methods.
Aggregation-induced emission (AIE) materials exhibit bright fluorescence in aggregates, making them suitable for bioimaging.

Purpose of the Study:

To review the applications of AIE-active materials in two-photon fluorescence imaging.
To highlight the benefits of AIE materials for advanced bioimaging techniques.
To discuss current challenges and future prospects in AIE-based two-photon bioimaging.

Main Methods:

Literature review focusing on AIE materials in two-photon fluorescence imaging.
Categorization of applications into fluorescence detection, in vitro cell imaging, ex vivo tissue imaging, and in vivo vascular imaging.
Analysis of representative examples showcasing AIE material performance.

Main Results:

AIE materials are increasingly utilized in two-photon fluorescence imaging due to their unique optical properties.
Successful applications demonstrated in various bioimaging contexts, including cellular and tissue levels.
AIE materials show strong resistance to photobleaching and high fluorescence quantum yields in aggregated states.

Conclusions:

AIE materials represent a promising class of compounds for advanced two-photon fluorescence bioimaging.
Further development is needed to address current challenges and fully exploit their potential.
AIE-based imaging holds significant potential for future biological and medical research.