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circRNA-miRNA-mRNA network in age-related macular degeneration: From construction to identification

Yu Su1, Yuexiong Yi2, Lu Li1

  • 1Eye Center, Renmin Hospital of Wuhan University, Wuhan, Hubei province, 430060, PR China.

Experimental Eye Research
|December 31, 2020
PubMed

Insights

Researchers constructed a circRNA-miRNA-mRNA network to understand age-related macular degeneration (AMD) pathogenesis. They identified a key regulatory axis, hsa_circRNA7329/hsa-miR-9/SCD, potentially driving AMD through inflammation and angiogenesis.

Area of Science:

  • Ophthalmology
  • Molecular Biology
  • Genetics

Background:

  • Age-related macular degeneration (AMD) is a leading cause of vision loss.
  • The complex molecular mechanisms underlying AMD pathogenesis are not fully understood.

Purpose of the Study:

  • To construct a regulatory circRNA-miRNA-mRNA network to elucidate AMD pathogenesis.
  • To identify key regulatory axes involved in AMD development.

Main Methods:

  • Differential gene expression analysis of AMD datasets (GSE50195, GSE29801).
  • Construction of a circRNA-miRNA-mRNA network using Starbase database and RNA hybrid assays.
  • Identification of key regulatory axes via protein-protein network analysis and MCODE algorithm.

Main Results:

  • Identified 2920 and 1057 differentially expressed mRNAs.
  • Constructed a network with 303 circRNA, 4 miRNA, and 51 mRNA nodes.
  • Identified the hsa_circRNA7329/hsa-miR-9/SCD regulatory axis as a potential driver of AMD.

Conclusions:

  • The hsa_circRNA7329/hsa-miR-9/SCD axis may promote AMD by enhancing macrophage-mediated inflammation and pathologic angiogenesis.
  • Further investigation is required to fully elucidate the underlying molecular mechanisms.
Keywords:
Age-related macular degenerationBioinformaticsNeurodegeneration diseaseNoncoding RNA

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