Related Experiment Video
Updated: Nov 23, 2025
CRISPR-Cas9 Mediated Gene Deletion in Human Pluripotent Stem Cells Cultured Under Feeder-Free Conditions
Published on: November 1, 2024
Generation of PRMT6 homozygous knockout human embryonic stem cell lines
1Department of Endocrinology, Chang Zheng Hospital, Shanghai 200003, China; School of Biomedical Sciences, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong, China.
None:
PRMT6 is a type I protein arginine methyltransferase (PRMT) which participates in diverse biological processes. To facilitate the understanding of PRMT6 functions, we generated two independent homozygous PRMT6 knockout Human ESCs clones, PRMT6-KO-10 and PRMT6-KO-24, in human WA01 ES cells by CRISPR/Cas9. The pluripotency of both clones was verified by the presence of pluripotent markers, normal karyotypes, and differentiation potential in vivo whereas the lack of PRMT6 expression was demonstrated by sequencing and detection of the significant changes in specific histone methylation patterns.
