Cryphonectria nitschkei chrysovirus 1 with unique molecular features and a very narrow host range
Sabitree Shahi1, Sotaro Chiba2, Hideki Kondo1
1Institute of Plant Science and Resources, Okayama University, Kurashiki, Okayama, 710-0046, Japan.
Virology
|December 31, 2020
Summary
Cryphonectria nitschkei chrysovirus 1 (CnCV1) complete genome sequencing reveals unique molecular features and a narrow host range. This virus can transfer to related fungi but does not cause symptoms, indicating asymptomatic infections.
Area of Science:
- Mycology
- Virology
- Molecular Biology
Background:
- Cryphonectria nitschkei chrysovirus 1 (CnCV1) was previously identified in Japan.
- A decade ago, only a partial sequence of CnCV1 was available.
Purpose of the Study:
- To perform complete genome sequencing of CnCV1.
- To analyze the molecular features of CnCV1.
- To investigate the host range and transmission of CnCV1.
Main Methods:
- Complete sequencing of four genomic dsRNA segments.
- Analysis of open reading frames and untranslated regions.
- Lateral transfer experiments via coculturing, virion transfection, and protoplast fusion.
Main Results:
- CnCV1 genome exhibits unique features, including mini-cistrons, and common chrysovirus traits like CAA repeats.
- CnCV1 can be transferred to Cryphonectria nitschkei, C. radicalis, and C. naterciae.
- Infection is symptomless in new hosts, and the virus has a very narrow host range.
Conclusions:
- CnCV1 possesses distinct molecular characteristics within the Chrysoviridae family.
- The virus demonstrates a highly specific host range, limited to closely related fungal species.
- CnCV1 establishes asymptomatic infections in its hosts, highlighting potential for cryptic spread.
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