Stem cell number versus the fraction synthesizing DNA
Related Experiment Video
Updated: Sep 16, 2026

Enumeration of Neural Stem Cells Using Clonal Assays
Published on: October 4, 2016
1Department of Pathophysiology, Faculty of General Medicine, Charles University, Prague, Czechoslovakia.
Abstract:
It has been widely held that the fraction of spleen colony-forming units (CFU-S) in DNA synthesis is inversely correlated with CFU-S numbers. In 750 measurements the expected negative slope of the linear regression of CFU-S in S-phase on CFU-S was found to be significant only when the measurements from irradiated mice were pooled. In contrast, a significant positive correlation was observed when the measurements from normal controls were pooled. A hypothesis is offered to account for the variable relationship between CFU-S and CFU-S in S. The hypothesis fully recognizes the crucial role of both parameters in normal and regenerative hemopoiesis.
Related Concept Videos
DNA Damage can Stall the Cell Cycle
Multipotency of Hematopoietic Stem Cells
Source And Potency Of Stem Cells
Embryonic Stem Cells
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
Maintenance of the ES Cell State
Methods of Nuclear Reprogramming

