Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bioengineered nasal septum implant with 3D-printed silicone and chondrocyte-seeded fibrin hydrogel.

Regenerative biomaterials·2026
Same author

Ex situ heart perfusion: a novel model for drug validation and translation.

Frontiers in cardiovascular medicine·2026
Same author

Cardiovascular <i>LRP1</i> expression varies by anatomical site, with selective age- and sex-associated changes in human tissue datasets.

American journal of physiology. Heart and circulatory physiology·2026
Same author

Impact of Stiffness and Cell-Binding Motif Availability on the Cell-Specific Response to Collagen-Based Macromolecular Materials.

Biomacromolecules·2026
Same author

A clinically defined and xeno-free hydrogel system for regenerative medicine.

Materials futures·2026
Same author

Ejaculation Sparing Bladder Neck Incision for Young Men With Primary Bladder Neck Obstruction.

International journal of urology : official journal of the Japanese Urological Association·2026

Related Experiment Video

Updated: Nov 23, 2025

Construction of Defined Human Engineered Cardiac Tissues to Study Mechanisms of Cardiac Cell Therapy
11:51

Construction of Defined Human Engineered Cardiac Tissues to Study Mechanisms of Cardiac Cell Therapy

Published on: March 1, 2016

10.6K

Modulating hESC-derived cardiomyocyte and endothelial cell function with triple-helical peptides for heart tissue

Maria Colzani1, Jean-Daniel Malcor2, Emma J Hunter2

  • 1Department of Medicine and Wellcome - MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK.

Biomaterials
|January 1, 2021
PubMed
Summary

This study shows that mimicking cell-collagen interactions with triple-helical peptides (THPs) improves engineered cardiac tissue. THPs enhance cardiomyocyte and endothelial cell function for better myocardial repair applications.

Keywords:
Cardiac tissue engineeringCollagen biomaterialsPluripotent stem cellsRegenerative medicineTriple-helical peptides

More Related Videos

3D Human Myocardial Tissue Generation Using Melt Electrospinning Writing of Polycaprolactone Scaffolds and hiPSC-Derived Cardiac Cells
06:17

3D Human Myocardial Tissue Generation Using Melt Electrospinning Writing of Polycaprolactone Scaffolds and hiPSC-Derived Cardiac Cells

Published on: March 28, 2025

821
Preparation of Mesh-Shaped Engineered Cardiac Tissues Derived from Human iPS Cells for In Vivo Myocardial Repair
05:05

Preparation of Mesh-Shaped Engineered Cardiac Tissues Derived from Human iPS Cells for In Vivo Myocardial Repair

Published on: June 9, 2020

5.8K

Related Experiment Videos

Last Updated: Nov 23, 2025

Construction of Defined Human Engineered Cardiac Tissues to Study Mechanisms of Cardiac Cell Therapy
11:51

Construction of Defined Human Engineered Cardiac Tissues to Study Mechanisms of Cardiac Cell Therapy

Published on: March 1, 2016

10.6K
3D Human Myocardial Tissue Generation Using Melt Electrospinning Writing of Polycaprolactone Scaffolds and hiPSC-Derived Cardiac Cells
06:17

3D Human Myocardial Tissue Generation Using Melt Electrospinning Writing of Polycaprolactone Scaffolds and hiPSC-Derived Cardiac Cells

Published on: March 28, 2025

821
Preparation of Mesh-Shaped Engineered Cardiac Tissues Derived from Human iPS Cells for In Vivo Myocardial Repair
05:05

Preparation of Mesh-Shaped Engineered Cardiac Tissues Derived from Human iPS Cells for In Vivo Myocardial Repair

Published on: June 9, 2020

5.8K

Area of Science:

  • Biomaterials Science
  • Cardiovascular Research
  • Tissue Engineering

Background:

  • Engineered cardiac patches are crucial for myocardial repair after infarction.
  • These patches require cardiomyocytes (CMs) and supportive cells like endothelial cells (ECs) for vascularization and CM function modulation.
  • Native myocardium function relies on cell-specific interactions with collagen.

Purpose of the Study:

  • To investigate the role of cardiomyocyte and endothelial cell interactions with collagen in vitro.
  • To develop triple-helical peptides (THPs) that mimic these cell-collagen interactions.
  • To assess THP-functionalized biomaterials for improved engineered cardiac tissue assembly.

Main Methods:

  • Utilized human embryonic stem cell (hESC)-derived CMs and ECs.
  • Seeded cells on biomaterials functionalized with THPs to replicate cell-collagen interactions.
  • Evaluated cell proliferation, activity, maturation, and coordinated contraction.

Main Results:

  • THPs enhanced proliferation and activity of both CMs and ECs, individually and in co-culture.
  • THPs promoted CM maturation and enabled coordinated cellular contraction on collagen films.
  • Demonstrated the importance of collagen interactions in directing cellular responses.

Conclusions:

  • Cell-collagen interactions are critical for cellular responses in engineered cardiac tissues.
  • THP-functionalized biomaterials are effective tools for directing cell behavior.
  • This approach advances the development of engineered cardiac tissues for myocardial repair.