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Enzymic properties of intestinal aminopeptidase P: a new continuous assay
J Lasch1, R Koelsch, T Steinmetzer
1Institute of Biochemistry, Medical Faculty, Martin-Luther-University Halle, GDR.
FEBS Letters
|January 25, 1988
Abstract:
A continuous photometric assay of aminopeptidase P activity was developed which is based on a coupled enzymic assay with the substrate Gly-Pro-Pro-pNA and DPP IV as auxiliary enzyme. This assay was used to evaluate the kinetic parameters and inhibitory profile of intestinal brush border aminopeptidase P.
