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Occurrence of glucose polymer in undifferentiated PC12 cells
1Laboratory of Glycoconjugate Research, Mitsubishi-Kasei Institute of Life Sciences, Tokyo, Japan.
FEBS Letters
|January 25, 1988
Abstract:
A large glucose polymer was found, following pronase digestion, in PC12 pheochromocytoma cells metabolically labeled with [1-3H]galactose. The polymer was excluded from a Bio-Gel A-0.5 m column and adsorbed by immobilized concanavalin A-Sepharose from which it was eluted with 10 mM alpha-methylmannoside. Glucose was found to be the sole component monosaccharide. Except for those capable of degrading glycogen, no exo- or endo-glycosidases cleaved the polymer. This is the first report on the occurrence of a glucose polymer in undifferentiated PC12 cells.