Mitochondrion-targeting near-infrared fluorescent probe for detecting intracellular nanomolar level hydrogen sulfide...

Hydrogen sulfide (H₂S) typically plays biphasic biological roles in living organisms with subnormal H₂S exerting cytoprotective effects such as participating in cardioprotective signaling pathways while H₂S with higher-than-normal concentrations in localized tissues acting the opposite way such as inhibiting mitochondrial respiration. Such concentration-dependent biological and pathological roles of H₂S with the wide involvement of mitochondria and the elusive feature of H₂S definitely highlight the vital significance of fast and precise estimation of the physiological level of H₂S in specific microenvironments, particularly within cellular mitochondria. In this work, we developed a new type of fluorescent probe (QcyCHO) featured with H₂S-triggered off-to-on near-infrared (NIR) fluorescence conversion within ~ 10 min, limit of detection (LOD) down to 8.3 nM, and high recognition specificity over other similarly interfering species. The ideal mitochondrion-targeting ability, high recognition specificity over typical interfering substances and other physiologically relevant species, and the ability for mapping intracellular H₂S in living cells of QcyCHO probe were also unequivocally confirmed, which imply its potential for shedding light on the biology of H₂S and therapeutic development in H₂S-associated diseases by identifying the specific physiological stimuli inducing H₂S production and determining the levels of H₂S at the location and time of stimulation.