A smartphone-integrated ratiometric fluorescence sensor for visual detection of cadmium ions

A novel fluorescence sensing platform was fabricated for visual detection of cadmium ions (Cd²⁺) with excellent stability and portability. In this protocol, dual-emission ratiometric fluorescence probe were constructed based on silicon oxide-coated copper nanoclusters (CuNCs@SiO₂) as a signal reference and cadmium telluride quantum dots (CdTe QDs) as signal response, thereby greatly improving the accuracy of test results. The level of Cd²⁺ can be reported within a wide linear range from 0.010 mg·L^-1 to 2.0 mg·L^-1 with a sensitive detection limit of 1.1 μg·L^-1 (2.75 μg·kg^-1) and a quick sample-to-answer monitoring time of 6 min, which was quite qualified for regularly monitoring Cd²⁺. Moreover, aiming to attain portable analysis, the smartphone as colorimetric reader and analyzer were also utilized for rapidly analyzing Cd²⁺ by capturing the change in fluorescence color. Additionally, benefiting from the strong combination of 1, 10-phenanthroline (Phen) and Cd²⁺, the fluorescence probe showed excellent anti-interference activities for Cd²⁺ assay in complex oyster matrix. Overall, the sensing platform had significant stability, specificity and sensitivity, offering a promising potential for conveniently evaluating the quality of marine bivalves polluted with Cd²⁺.