PK15 cell line stably overexpressing IL2 enhances PCV2 replication

Area of Science:

• Veterinary Virology
• Molecular Biology
• Biotechnology

Background:

• Porcine circovirus type 2 (PCV2) causes economically significant porcine circovirus-associated diseases (PCVADs) in swine.
• Current PCV2 vaccine development requires substantial viral particle yields, which are limited by low in vitro propagation rates.
• Transient transfection methods for enhancing PCV2 yield are unstable across serial cell generations.

Purpose of the Study:

• To develop a stable cell line for enhanced porcine circovirus type 2 (PCV2) replication.
• To improve the efficiency and stability of producing viral particles for PCV2 vaccine development.

Main Methods:

• Construction of a PK15 cell line with stable porcine interleukin-2 (IL-2) expression using lentivirus transfection.
• Evaluation of PCV2 replication efficiency in the engineered cell line compared to control cells.

Main Results:

• The transgenic PK15 cell line demonstrated stable expression of porcine IL-2.
• Significantly enhanced PCV2 replication was observed in the IL-2 expressing transgenic cell line.
• The stable expression of IL-2 led to higher viral yields.

Conclusions:

• A stably engineered PK15 cell line expressing porcine IL-2 promotes enhanced PCV2 replication.
• This transgenic cell line represents a promising platform for efficient and stable PCV2 vaccine production.
• The findings contribute to overcoming limitations in current PCV2 vaccine manufacturing.