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Analysis of gene expression of prostaglandin EP4 receptor in canine osteosarcoma
Margaret L Musser1, Austin K Viall1, Rachel L Phillips1
1Department of Veterinary Clinical Sciences (Musser, Johannes) and Department of Veterinary Pathology (Viall, Phillips, Hostetter), Iowa State University College of Veterinary Medicine, 1800 Christensen Drive, Ames, Iowa 50011-1134, USA.
Abstract:
In many human cancers, the expression of the prostaglandin receptor EP4 (EP4R) is associated with the development of malignancy and a poor prognosis. The expression of EP4R has not yet been evaluated in canine tumors. The objective of this study was to characterize the messenger RNA (mRNA) expression of EP4R in canine osteosarcoma (OSA). Gene expression of EP4R was evaluated using RNA in-situ hybridization (RNAscope). In all canine OSA samples evaluated, strong universal positive expression of EP4R was identified. Gene expression was significantly higher in OSA tissue samples than in normal nasal turbinate bone, possibly implicating EP4R in the pathogenesis of canine OSA.
