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Related Concept Videos

Hybrid Zones02:29

Hybrid Zones

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Hybrid zones are narrow regions where two closely related species interact, mate, and produce hybrids. Relative to either parent species, hybrids may possess distinct phenotypic or genetic differences that impact their survival and reproductive success. The genetic variances introduced by hybridization influence species diversity and speciation processes within the hybrid zone.
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The mathematical expression known as the wave function, ψ, contains information about each orbital and the wavelike properties of electrons in an isolated atom. When atoms are bound together in a molecule, the wave functions combine to produce new mathematical descriptions that have different shapes. This process of combining the wave functions for atomic orbitals is called hybridization and is mathematically accomplished by the linear combination of atomic orbitals. The new orbitals that...
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Southern Blot02:57

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Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
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In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
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Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
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Non-radioactive in situ Hybridization Protocol Applicable for Norway Spruce and a Range of Plant Species
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Northern Hybridization.

Michael R Green, Joseph Sambrook

    Cold Spring Harbor Protocols
    |January 5, 2021
    PubMed
    Summary

    This study details a method for detecting specific RNA using labeled probes hybridized to membranes. The technique allows for sensitive RNA detection and membrane reuse after probe stripping.

    Area of Science:

    • Molecular Biology
    • Biochemistry
    • Genetics

    Background:

    • Hybridization techniques are crucial for detecting specific nucleic acid sequences.
    • Membrane-based assays are widely used for RNA detection and analysis.

    Purpose of the Study:

    • To describe a robust method for locating specific RNA species using labeled probes.
    • To outline the steps involved in probe hybridization, detection, and analysis on a membrane.

    Main Methods:

    • Immobilization of RNA samples onto a membrane.
    • Hybridization with a labeled probe specific to the target RNA.
    • Washing to remove unbound probe and imaging for detection.

    Main Results:

    • Successful localization of target RNA species through specific probe binding.

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  • Demonstration of the ability to strip probes for subsequent re-use of the membrane.

    • Conclusions:

    • The described method provides a reliable approach for sensitive RNA detection.
    • The reusability of membranes enhances the efficiency and cost-effectiveness of hybridization experiments.