Considerations for Membrane Protein Purification
Related Experiment Video
Updated: Nov 23, 2025

08:37
Purification of the Sarco-Endoplasmic Reticulum Ca2+-ATPase from Rabbit Muscle
Published on: March 21, 2025
629
Cold Spring Harbor Protocols
|January 5, 2021
Abstract:
Isolating membrane proteins from their native cells while maintaining structural and functional integrity is challenging. Many detergents have been developed over the years that interact favorably with membrane proteins and mimic the physical properties of the lipid bilayer. Choosing the appropriate detergent is crucial for the successful extraction of a protein in its properly folded, active conformation.

