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Related Experiment Video

Updated: Nov 23, 2025

3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells
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Left-handed DNA-PAINT for improved super-resolution imaging in the nucleus.

H J Geertsema1, G Aimola2, V Fabricius1

  • 1Institute of Chemistry and Biochemistry, Department for Biology, Chemistry and Pharmacy, Freie Universität Berlin, Berlin, Germany.

Nature Biotechnology
|January 5, 2021
PubMed
Summary
This summary is machine-generated.

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Left-handed DNA (L-DNA) oligomers reduce background noise in super-resolution imaging. L-DNA-PAINT offers high specificity and multiplexing for clearer nuclear target visualization.

Area of Science:

  • Molecular biology
  • Biophysics
  • Microscopy

Background:

  • DNA point accumulation in nanoscale topography (DNA-PAINT) is a super-resolution imaging technique.
  • Cellular DNA in the nucleus can interfere with DNA-DNA hybridization, increasing background signal.
  • Existing DNA-PAINT methods face challenges in imaging nuclear targets due to this interference.

Purpose of the Study:

  • To develop a novel DNA-PAINT method for improved imaging of nuclear targets.
  • To overcome the limitations of right-handed DNA (R-DNA) interference in cellular imaging.
  • To enhance specificity and reduce background noise in super-resolution microscopy.

Main Methods:

  • Introduction of left-handed DNA (L-DNA) oligomers.
  • Demonstration of L-DNA's inability to hybridize with natural R-DNA.

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  • Application of L-DNA in DNA-PAINT for nuclear target imaging.

    • Main Results:

    • L-DNA-PAINT exhibits equivalent specificity and multiplexing capabilities to R-DNA-PAINT.
    • L-DNA-PAINT significantly reduces background signal in nuclear imaging.
    • Clearer visualization of nuclear targets is achieved using L-DNA-PAINT.

    Conclusions:

    • Left-handed DNA (L-DNA) is a viable alternative for DNA-PAINT in nuclear imaging.
    • L-DNA-PAINT enhances super-resolution microscopy by minimizing background interference.
    • This method offers improved resolution and multiplexing for cellular targets.