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Instrument Calibration

Instrument Calibration

Instrument calibration is essential for ensuring that instruments produce accurate and consistent results. It is vital in manufacturing, healthcare, testing laboratories, and scientific research. Calibration processes are specific to each instrument and help enhance data accuracy. Each instrument has a unique calibration process tailored to its design and function to improve data accuracy.
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Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...

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Updated: Nov 23, 2025

Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA

Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA

Published on: September 10, 2013

Calibrating analytical ultracentrifuges.

Huaying Zhao¹, Ai Nguyen¹, Samuel C To¹

¹Dynamics of Macromolecular Assembly Section, Laboratory of Cellular Imaging and Macromolecular Biophysics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA.

European Biophysics Journal : EBJ

|January 5, 2021

Summary

This summary is machine-generated.

Analytical ultracentrifugation (AUC) calibration using external standards significantly reduces systematic errors. This method ensures precise and consistent macromolecular measurements across different instruments and laboratories.

Keywords:

Analytical ultracentrifugation Instrument calibration Sedimentation velocity Stokes radius

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Area of Science:

Biophysical characterization
Analytical chemistry
Macromolecular science

Background:

Analytical ultracentrifugation (AUC) measures macromolecular properties based on first principles.
Systematic errors in AUC instruments affect measurements of mass, sedimentation, and diffusion coefficients.
Previous work introduced reference materials and protocols for AUC error detection and correction.

Purpose of the Study:

To detail the steps for calibrating data dimensions in AUC.
To demonstrate the calibration process on multiple AUC instruments.
To validate calibration using NISTmAb monomer sedimentation coefficient measurements.

Main Methods:

External calibration using independently verified standards.
Calibration of optical detection magnification, sedimentation time, and temperature measurement.
Demonstration on three AUC instruments with absorbance and interference detection.

Main Results:

Uncorrected sedimentation coefficients varied widely (6.22–6.61 S).
Proper calibration reduced the standard deviation of sedimentation coefficients tenfold.
Calibrated measurements showed a relative standard deviation of 0.2% and mean error of 0.07%.

Conclusions:

External calibration dramatically improves AUC precision and inter-laboratory consistency.
Calibrated AUC results achieve accuracy comparable to statistical and single-instrument repeatability.
Standardized calibration protocols are essential for reliable macromolecular characterization via AUC.