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Updated: Nov 23, 2025

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High-Specific Fluorescence Probe for SO32- Detection and Bioimaging.

Jianbin Chao1, Jiamin Zhao2,3, Yongbin Zhang4

  • 1Scientific Instrument Center, Shanxi University, Taiyuan, 030006, People's Republic of China. chao@sxu.edu.cn.

Journal of Fluorescence
|January 5, 2021
PubMed
Summary

Researchers developed a new pyrene derivative probe, PPA, for sensitive sulfite (SO3^2-) detection. This probe offers a simple, specific, and efficient method for monitoring sulfite levels in biological systems, aiding disease research.

Keywords:
BioimagingFluorescent probeMichael additionSO3 2 −

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Chemical Biology

Background:

  • Sulfite (SO3^2-) is crucial in physiological processes.
  • Abnormal sulfite levels are linked to various diseases affecting multiple organ systems.
  • Efficient sulfite detection methods are essential for health monitoring and disease diagnosis.

Purpose of the Study:

  • To design and synthesize a novel pyrene derivative probe, PPA, for selective sulfite detection.
  • To investigate the reaction mechanism and performance characteristics of the PPA probe.
  • To evaluate the applicability of PPA for biological imaging of sulfite in cells.

Main Methods:

  • Synthesis of the pyrene derivative (E)-4-(3-oxo-3-(pyren-1-yl)prop-1-en-1-yl)phenyl acrylate (PPA).
  • Characterization of the PPA probe's response to sulfite using spectroscopic analysis.
  • Confirmation of the reaction mechanism via HRMS and FTIR analysis.
  • Determination of the linear detection range and limit of detection for sulfite.

Main Results:

  • PPA was successfully synthesized with simple steps and demonstrated excellent anti-interference capabilities.
  • The probe exhibited a specific response to sulfite, attributed to Michael addition.
  • A linear detection range of 0-100 μM and a low limit of detection (0.17 μM) were achieved.
  • PPA was successfully applied for biological imaging of sulfite in HepG2 cells.

Conclusions:

  • The PPA probe provides an efficient and selective method for sulfite detection.
  • The probe's performance characteristics make it suitable for quantitative sulfite analysis.
  • PPA holds potential for in vivo monitoring of sulfite levels in biological contexts.