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Updated: Nov 23, 2025

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Published on: March 19, 2018

[Preface for microbiome sequencing and analysis].

Yunfeng Duan¹, Baoli Zhu¹

¹Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.

Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology

|January 5, 2021

Summary

This summary is machine-generated.

Microbiome sequencing and analysis are essential for understanding microbial communities across diverse environments and applications. This collection of papers reviews current methods, databases, and future trends in microbiome research.

Keywords:

16S rRNA amplicon sequencing gene sequencing metagenome microbes microbiome

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Last Updated: Nov 23, 2025

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Published on: March 19, 2018

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Published on: October 15, 2019

Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere

Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere

Published on: May 2, 2018

Area of Science:

Microbiology
Genomics
Bioinformatics

Background:

Microbes are crucial commensals in humans, animals, and plants, inhabiting diverse environments like soil and water.
Microbiome analysis is a fundamental research technique with broad applications.
Rapid advancements in microbiome sequencing and analysis technologies have emerged.

Purpose of the Study:

To review the current status, development, and application prospects of microbiome sequencing and analysis.
To compile a collection of 16 papers covering various aspects of microbiome research.
To provide insights into the future trends of microbiome sequencing and analysis.

Main Methods:

Review of 16 papers on microbiome sequencing and analysis.
Inclusion of topics such as sample preservation, single microbe genome analysis, and feature analysis in specific habitats.
Discussion of microbiome-related databases, algorithms, and expert consensus.

Main Results:

The collection covers sample processing, genome sequencing, and microbiome feature analysis.
It includes insights into microbiome databases, algorithms, and expert consensus.
The review highlights the development trends in the field.

Conclusions:

Microbiome sequencing and analysis are vital across human health, environmental, food, agricultural, and animal husbandry sectors.
The reviewed works aim to promote the rapid development of the microbiome industry and scientific research.
This compilation serves as a reference for the healthy development of related industries.