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Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing
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[Expert consensus on microbiome sequencing and analysis].

Yunfeng Duan1, Shengyue Wang2, Yubao Chen3

  • 1Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.

Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
|January 5, 2021
PubMed
Summary

The microbiome field is rapidly growing but lacks standardized methods and data. This expert consensus aims to guide research and industry practices in China for regulated development.

Keywords:
expert consensushigh-throughput gene sequencingmicrobiomemicrobiome projectnational standards

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Area of Science:

  • Microbiome research
  • Life sciences
  • Environmental science
  • Medicine

Background:

  • Microbiome research has surged globally in the last decade, becoming a focus in life sciences, environmental science, and medicine.
  • International microbiome projects and significant investment highlight the field's strategic importance and rapid growth.
  • Current research faces challenges due to a lack of unified standards in sequencing, analysis, technology, and data management.

Purpose of the Study:

  • To address the lack of standardized practices in China's burgeoning microbiome field.
  • To provide a unified expert consensus for regulating microbiome research and industrial applications.
  • To establish a technical basis for national functional departments and promote industry self-discipline.

Main Methods:

  • Development of an expert consensus through collaboration among microbiome experts and practitioners.
  • Review of existing technologies, methods, and data standards in microbiome research.
  • Analysis of the current landscape of microbiome research and industrial application in China.

Main Results:

  • Identified a critical need for standardized microbiome technologies, programs, and data management in China.
  • Highlighted the impracticality of some existing general standards, leading to data integration issues and resource waste.
  • Noted the absence of national microbiome standards and the ongoing preparation of a national microbiome project in China.

Conclusions:

  • The developed expert consensus provides essential guidance for domestic scientific research and industrial institutions.
  • It serves as a reference for national functional departments, aiming to protect legitimate businesses and prevent market disruption.
  • The consensus promotes standardized practices, fostering the healthy and regulated development of China's microbiome industry.