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Related Concept Videos

RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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Updated: Nov 23, 2025

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
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Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

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[A new quantitative 16S rRNA amplicon sequencing method].

Na Han1,2, Xianhui Peng1,2, Tingting Zhang1,2

  • 1Department of Bioinformatics, State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.

Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
|January 5, 2021
PubMed
Summary
This summary is machine-generated.

We developed a quantitative 16S rRNA sequencing method to improve human gut microbiota analysis accuracy. This new approach enhances bacterial population resolution and reduces experimental variability for more reliable results.

Keywords:
16S rRNAampliconinternal markerquantitativerandom tag

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Area of Science:

  • Microbiology
  • Genomics
  • Bioinformatics

Background:

  • 16S rRNA amplicon sequencing is crucial for studying gut microbiota and pathogen detection.
  • Current methods have limitations in bacterial population resolution (genus level) and are susceptible to experimental variations.
  • Factors like sample concentration, PCR cycles, and primers influence sequencing outcomes.

Purpose of the Study:

  • To develop a more accurate and reliable quantitative 16S rRNA amplicon sequencing method.
  • To overcome the resolution limitations of existing techniques for bacterial population analysis.
  • To minimize the impact of experimental variables on gut microbiota profiling.

Main Methods:

  • Development of a novel quantitative 16S rRNA amplicon sequencing approach.
  • Integration of random tag and internal marker techniques.
  • Application to human gut microbiota samples.

Main Results:

  • Significantly improved accuracy in human gut microbiota composition analysis.
  • Reduced variability and impact of experimental operations on sequencing results.
  • Enhanced comparability between 16S rRNA sequencing and other molecular methods.

Conclusions:

  • The new quantitative method offers superior resolution and accuracy for bacterial population studies.
  • This technique provides a more robust and reproducible approach to gut microbiome analysis.
  • The findings facilitate more reliable clinical and research applications of 16S rRNA sequencing.