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Real Time Monitoring of Intracellular Bile Acid Dynamics Using a Genetically Encoded FRET-based Bile Acid Sensor
Published on: January 4, 2016
10.2K
[Bile acid detection by biosensors-a review]
Enpeng Dong1,2, Junping Zheng1, Hongtao Liu1
1School of Basic Medicine, Hubei University of Chinese Medicine, Wuhan 430065, Hubei, China.
Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
|January 5, 2021
Summary
Accurate detection of bile acids is crucial for understanding diseases. Current methods face challenges, but advancements in biosensors offer promising future prospects for precise bile acid quantification.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Clinical Diagnostics
Background:
- Bile acids are vital for lipid absorption and influence diseases via gut microbiota, immunity, and metabolism.
- Quantitative detection of bile acids is essential due to their complex composition and variable bioactivity.
- Existing analytical methods for bile acids are limited in accuracy and scope.
Purpose of the Study:
- To review current research progress in bile acid analysis.
- To highlight the limitations of conventional bile acid detection methods.
- To emphasize the potential of emerging bile acid biosensors.
Main Methods:
- Review of spectrophotometry, thin-layer chromatography, and liquid/gas chromatography.
- Analysis of liquid/gas chromatography-mass spectrometry techniques.
- Exploration of novel bile acid biosensor development.
Main Results:
- Conventional methods for bile acid quantification from various sources (serum, bile, urine, feces) have inherent limitations.
- Significant progress has been made in developing advanced analytical techniques.
- Bile acid biosensors show considerable promise for future applications.
Conclusions:
- Accurate bile acid detection is critical for pharmacological research, clinical diagnosis, and disease prevention.
- Overcoming current analytical challenges is necessary for reliable bile acid quantification.
- Biosensor technology represents a promising avenue for improved bile acid analysis.
