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Related Experiment Video

Updated: Nov 23, 2025

Analyzing Platelet Subpopulations by Multi-color Flow Cytometry
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Analyzing Platelet Subpopulations by Multi-color Flow Cytometry

Published on: June 10, 2025

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Platelet mass cytometry: Optimization of sample, reagent, and analysis parameters.

Benjamin E J Spurgeon1, Alan D Michelson1, Andrew L Frelinger1

  • 1Center for Platelet Research Studies, Dana-Farber/Boston Children's Cancer and Blood Disorders Center, Harvard Medical School, Boston, Massachusetts, USA.

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
|January 5, 2021
PubMed
Summary

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Markers for platelet function testing by flow cytometry: where are we now? A clinical perspective.

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Seminars in thrombosis and hemostasis·2024

Mass cytometry enables detailed analysis of platelet subsets, revealing how sample preparation impacts results. This method improves understanding of platelet heterogeneity in health and disease.

Area of Science:

  • Hematology
  • Immunology
  • Cell Biology

Background:

  • Platelets are crucial for hemostasis, immunity, and inflammation.
  • Platelets exhibit heterogeneity in size, age, and receptor expression, suggesting specialized functions.
  • Existing flow cytometry methods for platelet subset identification have limitations.

Purpose of the Study:

  • To detail best practices for platelet sample preparation, processing, and analysis using mass cytometry.
  • To optimize mass cytometry for robust platelet subset identification.
  • To map platelet heterogeneity in resting and stimulated states.

Main Methods:

  • Utilized mass cytometry with a 14-metal-tagged antibody panel targeting platelet surface markers.
  • Evaluated the impact of anticoagulant choice, antibody cocktail storage, and sample processing on platelet phenotype and function.
Keywords:
biomarkersblood plateletscluster analysisflow cytometryimmunophenotypingplatelet activationplatelet function tests

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  • Optimized sample density and instrument setup for maximal platelet transmission.
  • Applied panel design, compensation, clustering, and dimension reduction for analysis.

    • Main Results:

    • Anticoagulant choice significantly alters platelet phenotype and function.
    • Reproducibility is critically dependent on antibody cocktail storage and sample processing.
    • Optimized sample density and instrument settings enhance platelet detection.
    • Mass cytometry effectively maps platelet heterogeneity across different activation states.

    Conclusions:

    • Mass cytometry is a powerful tool for dissecting platelet heterogeneity.
    • Standardized protocols are essential for reliable platelet subset analysis.
    • Understanding platelet subsets can provide insights into disease mechanisms.