Preparation, activity, and antioxidant mechanism of rice bran polysaccharide

Rice bran polysaccharide has a wide range of physiological functions, which has aroused great interest of scientists at home and abroad. It is an ideal healthcare product. The polysaccharide was extracted from defatted rice bran with water. It was subjected to protein removal with the Sevag reagent. The chemical structure of rice bran polysaccharide was analyzed by infrared spectroscopy and nuclear magnetic resonance spectroscopy. The polysaccharide significantly increased the content of superoxide dismutase and catalase in the serum, liver and spleen of mice, and decreased the content of malondialdehyde in the serum, liver and spleen to a certain extent. The polysaccharide could upregulate the expression of nuclear factor E₂-related factor 2 and its downstream antioxidant factors NQ01 and HO-1, and enhance the activity of the antioxidant response element luciferase.