Related Experiment Video
Updated: Nov 22, 2025

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses
Published on: December 29, 2015
Study on the Dynamic Proliferation of JEV in BHK-21 Cells
Fuliang Zhang1,2, Jun Luo3, Man Teng3
1College of Veterinary Medicine, Northwest A & F University, Yangling, China.
Introduction:
Epidemic Japanese encephalitis is one of the most important zoonotic diseases that cause central nervous system damage. The vaccination has become the most effective and economical measure for its control. Hence, real-time monitoring of Japanese encephalitis virus (JEV) proliferation is crucial to optimize virus inoculation, culturing conditions, and virus harvest time.
Methods:
The proliferation dynamics of JEV in BHK-21 cells was studied by combining the established quantitative PCR method with the conventional TCID50 assay in this study.
Results:
The proliferation curve determined by the 2 methods has a definite parallel relationship, but the quantitative real-time PCR method (4 h) is faster and more sensitive than the TCID50 method (3-4 days). The determination results of TCID50 showed that the highest viral titer was 105.44 TCID50/0.1 mL and 104.86 TCID50/0.1 mL in cell suspension and culture supernate, respectively, while the virus RNA copies reached the peak at 1.0 × 107.5 copies/µL and 1.0 × 105.6 copies/µL in cell suspension and culture supernate, respectively.
Conclusion:
The comprehensive analysis showed that the best time for JEV proliferation in BHK-21 cell was 60 h post infection.
More Related Videos
Related Concept Videos
Cells Coordinate Growth and Proliferation
Proliferative Phase
Notably, the stratum basale, the basal layer of the endometrium, including the basal parts of the uterine glands, remains unaffected by menstruation. Stem cells in this layer undergo mitosis, regenerating the stratum functionalis and thickening the...
Multipotency and Niche of Bulge Stem Cell
Abnormal Proliferation
In-vitro Mutagenesis
Cell Motility through Blebbing
Blebbing Through the Matrix
In multicellular...

