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Novel Molecular Markers Linked to Pseudomonas aeruginosa Epidemic High-Risk Clones

Wedad Nageeb1, Dina H Amin2, Zuhair M Mohammedsaleh3

  • 1Medical Microbiology and Immunology Department, Faculty of Medicine, Suez Canal University, Ismailia 41111, Egypt.

Antibiotics (Basel, Switzerland)
|January 6, 2021
PubMed

Insights

High-risk Pseudomonas aeruginosa clones, linked to antibiotic resistance and severe illness, owe their success to mobile genetic elements and novel chromosomal variants. Identifying these markers aids infection control.

Area of Science:

  • Microbiology
  • Genomics
  • Epidemiology

Background:

  • Pseudomonas aeruginosa exhibits a panmictic-epidemic population structure, characterized by prevalent high-risk clones.
  • These successful clones are associated with increased virulence, antibiotic resistance, and patient morbidity.
  • The spread of mobile genetic elements is a known factor contributing to the success of these lineages.

Purpose of the Study:

  • To identify additional molecular markers responsible for the global success of high-risk Pseudomonas aeruginosa clones.
  • To investigate the role of chromosomal variants beyond mobile genetic elements.

Main Methods:

  • Analysis of 528 complete Pseudomonas aeruginosa genome sequences.
  • Population structure assessed using Multilocus Sequence Typing (MLST).
  • Strain relationships and diversity analyzed with geoBURST Full Minimum Spanning Tree (MST) and hierarchical clustering.
  • Phylogenetic tree construction using the Unweighted Pair Group Method with Arithmetic mean (UPGMA) algorithm.
  • Examination of known resistance markers and identification of novel markers.

Main Results:

  • A novel panel of molecular markers associated with high-risk clones was identified, including chromosomal variants in genes such as armR, ampR, nalC, nalD, mexZ, gyrA, and parC.
  • These novel markers include specific mutations like gyrAT83I, gyrAD87N, nalCE153Q, nalCS46A, parCS87W, parCS87L, ampRG283E, ampRM288R, pmrALeu71Arg, pmrBGly423Cys, nuoGA890T, pstBE89Q, phoQY85F, arnAA170T, arnDG206C, and gidBE186A.
  • Chromosomal variants in membrane proteins and efflux pump regulators, in addition to mobile genetic elements, are crucial for the success of high-risk clones.

Conclusions:

  • Chromosomal variants play a significant role in the success of high-risk Pseudomonas aeruginosa clones, complementing the impact of mobile genetic elements.
  • The identified novel molecular markers provide valuable targets for understanding and tracking these successful, potentially dangerous clones.
  • Early detection of risk-associated markers through molecular surveillance is essential for implementing enhanced infection-control measures.
Keywords:
MLSTPseudomonas aeruginosa high-risk clonesantibiotic resistanceclonal successmolecular markerspopulation structurevirulence

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