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Updated: Jun 16, 2026
Determination of Total Lipid and Lipid Classes in Marine Samples
Published on: December 11, 2021
Fatty Acid Composition of the Lipids from Atlantic Salmon-Comparison of Two Extraction Methods without Halogenated
Jordan T Nechev1, Guro K Edvinsen1, Karl-Erik Eilertsen1
1The Norwegian College of Fishery Science, The Faculty of Biosciences, Fisheries and Economics, UiT The Arctic University of Norway, N-9037 Tromso, Norway.
Abstract:
The scope of this paper was to apply two recently developed methods for lipid extraction: the methyl tert-butyl ether (MTBE) method and the BUME method. These two methods do not include halogenated solvents, which makes them less hazardous to the environment, less toxic, and needed in less volume compared to the standard methods for lipid extraction. Fatty acid composition of the lipids from Atlantic salmon (Salmo salar Linnæus, 1758) was obtained by both procedures. The methods were effective and thirty-three fatty acids were identified. The amounts of the omega-3 polyunsaturated fatty acids obtained by the MTBE method were found to be similar to the overall mean values observed in farmed salmon. The yield of the total lipids obtained by the BUME method was 13% lower. Although the methods involved different solvents, they showed similar fatty acids profile of the lipids from Atlantic salmon. Both methods were validated and some practical challenges were discussed.
