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Related Experiment Video

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The p24 Complex Contributes to Specify Arf1 for COPI Coat Selection.

Susana Sabido-Bozo1,2, Ana Maria Perez-Linero1,2, Javier Manzano-Lopez1,3

  • 1Department of Cell Biology, Faculty of Biology, University of Seville, 41012 Seville, Spain.

International Journal of Molecular Sciences
|January 6, 2021
PubMed
Summary

The p24 complex in yeast cis-Golgi promotes COPI vesicle budding by enhancing guanine nucleotide exchange factor Gea1 (GEF) binding to COPI. This facilitates Arf1 (ADP-ribosylation factor 1) activation for selective coat assembly.

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Golgi trafficking relies on Arf1 (ADP-ribosylation factor 1) GTPase, activated by guanine nucleotide exchange factors (GEFs).
  • Different GEFs (Gea1, Gea2, Sec7) activate Arf1 at distinct Golgi compartments, directing the assembly of specific vesicle coats like COPI and clathrin/adaptor.
  • A key question is how Arf1 recruits different coats based on its activation site.

Purpose of the Study:

  • To investigate the role of the p24 complex in programming Arf1 activation for selective COPI coat assembly at the cis-Golgi.
  • To elucidate the mechanism by which the p24 complex influences the interaction between Gea1 and COPI.

Main Methods:

  • Yeast genetics and biochemistry techniques were employed.
  • Analysis of protein-protein interactions and membrane association was performed.

Main Results:

  • The p24 complex enhances the association of Gea1 with COPI, independent of Arf1 activation.
  • The p24 complex increases COPI's membrane association.
  • The p24 complex facilitates the interaction between Arf1 and its COPI effector.

Conclusions:

  • The p24 complex acts as a crucial factor in directing Arf1 activation by Gea1 towards COPI coat assembly at the cis-Golgi.
  • This mechanism ensures the selective recruitment of COPI for retrograde cargo sorting in the Golgi.
  • The findings provide insight into the compartment-specific regulation of vesicle formation.