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[Plaque assay for Chlamydia psittaci in tissue samples (author's transl)]
Annales De Microbiologie
|July 1, 1977
Summary
Researchers compared two Chlamydia psittaci isolation methods. The plaque assay on McCoy cells proved more sensitive and reproducible than cytoplasmic inclusion staining in HeLa 229 cells for quantifying Chlamydiae in mouse spleen tissue.
Area of Science:
- Veterinary Microbiology
- Infectious Diseases
- Cell Biology
Background:
- Chlamydia psittaci (var, ovis) is an important veterinary pathogen.
- Accurate isolation and quantitation of Chlamydiae are crucial for disease diagnosis and research.
Purpose of the Study:
- To compare the efficacy of two direct isolation methods for Chlamydia psittaci from mouse spleen.
- To evaluate the sensitivity and reproducibility of plaque assay versus cell staining for Chlamydial detection.
Main Methods:
- Direct isolation of Chlamydia psittaci from mouse spleen.
- Parallel application of plaque assay on McCoy cells and staining of cytoplasmic inclusions in HeLa 229 cells.
Main Results:
- A significant difference in isolation frequency was observed between the two methods.
- The plaque assay demonstrated high sensitivity and reproducibility.
- Cytoplasmic inclusion staining showed lower sensitivity in this comparative study.
Conclusions:
- The plaque assay on McCoy cells is a superior method for sensitive and reproducible isolation and quantitation of Chlamydia psittaci from tissue samples.
- This technique offers a valuable tool for Chlamydial investigations requiring accurate quantification.